概述

  • 产品名称
    Anti-LIM kinase 2抗体
    参阅全部 LIM kinase 2 一抗
  • 描述
    兔多克隆抗体to LIM kinase 2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 294 and 665 of Human LIM kinase 2 (AAH13051).

  • 阳性对照
    • Molt-4 and Raji cell lines. 293T whole cell lysate. Paraffin-embedded CA922 xenograft.

性能

应用

Our Abpromise guarantee covers the use of ab97766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能
    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • 组织特异性
    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • 细胞定位
    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

图片

  • Western blot - Anti-LIM kinase 2 antibody (ab97766)
    Western blot - Anti-LIM kinase 2 antibody (ab97766)
    Anti-LIM kinase 2 antibody (ab97766) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 78 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM kinase 2 antibody (ab97766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM kinase 2 antibody (ab97766)
    ab97766, at 1/500, staining paraffin-embedded CA922 xenograft by Immunohistochemistry.

文献

This product has been referenced in:
  • Wang S  et al. BMPR2 promotes invasion and metastasis via the RhoA-ROCK-LIMK2 pathway in human osteosarcoma cells. Oncotarget 8:58625-58641 (2017). Read more (PubMed: 28938584) »

See 1 Publication for this product

发表研究结果有使用 ab97766？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交评价 提交问题

There are currently no Customer reviews or Questions for ab97766.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册