Anti-LIM kinase 2抗体(ab70116)

概述

  • 产品名称Anti-LIM kinase 2抗体
    参阅全部 LIM kinase 2 一抗
  • 描述
    兔多克隆抗体to LIM kinase 2
  • 特异性ab70116 detects endogenous levels of total LIM kinase 2 protein.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human LIM kinase 2

  • 阳性对照
    • HeLa cells, HepG2 and HUVEC cell extracts and human heart tissue.

性能

应用

Our Abpromise guarantee covers the use of ab70116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • 组织特异性Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on serine and/or threonine residues by ROCK1.
  • 细胞定位Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Anti-LIM kinase 2 antibody 图像

  • ab70116 at 1/500 dilution staining LIM kinase 2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70116 at 1/50 dilution staining LIM kinase 2 in human heart by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-LIM kinase 2 antibody (ab70116) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : HUVEC cell extracts
    Lane 3 : HepG2 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 72 kDa
    Observed band size : 72 kDa

Anti-LIM kinase 2 antibody (ab70116)参考文献

ab70116 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"