Anti-LIM Kinase 1 (phospho T508)抗体(ab38508)

概述

  • 产品名称
    Anti-LIM Kinase 1 (phospho T508)抗体
    参阅全部 LIM Kinase 1 一抗
  • 描述
    兔多克隆抗体to LIM Kinase 1 (phospho T508)
  • 特异性
    This antibody is specific for LIM Kinase only when phosphorylated at threonine 508. It recognizes both LIMK1 and LIMK2 from human, mouse, and rat.
  • 经测试应用
    适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human LIM Kinase around the phosphorylation site of threonine 508.

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue slides. WB: COLO and MCF7 cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab38508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.
ICC/IF 1/100. PubMed: 19208621

靶标

  • 功能
    Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • 组织特异性
    Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • 疾病相关
    Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • 细胞定位
    Cytoplasm. Cell projection > growth cone.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.1.37 antibody
    • LIM domain containing protein kinase antibody
    • LIM domain kinase 1 antibody
    • LIM kinase antibody
    • LIM motif containing protein kinase antibody
    • LIMK 1 antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 (phospho T508) antibody 图像

  • ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : untreated.

    Right image : treated with phosphopeptide.

  • All lanes : Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) at 1/500 dilution

    Lane 1 : Extract of COLO cells + peptide.
    Lane 2 : Extract of COLO cells.
    Lane 3 : Extract of MCF7 cells.

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 73 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Lanes can be loaded with 5-30µg of total protein. The arrow next to the image points to LIM Kinase (phospho T508).
  • ab38508 staining LIM Kinase 1 in mouse brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    bdKO mice were anesthetized and transcardially perfused with PBS, followed by 4% paraformaldehyde (PFA) prior to brain dissection. Brains were then postfixed in 4% PFA for 2 days, embedded in paraffin and sectioned.

Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)参考文献

This product has been referenced in:
  • Tsai CS  et al. Thrombomodulin regulates monocye differentiation via PKCd and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep 6:38421 (2016). WB ; Human . Read more (PubMed: 27910925) »
  • Ray S  et al. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell 25:2393-407 (2014). Read more (PubMed: 24966172) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 27 2011

Thank you for your patience. I have been in contact with the originator of this antibody, and below is the detailed antigen retrieval protocol. The method of antigen retrieval was used for IHC-P as following: Deparaffinization 1. Incubate slide a...

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Thank you for your enquiry. From data of recent testing, this antibody recognizes both LIMK1 and LIMK2 from human, mouse, and rat. I have clarified this on the datasheet; should you have further questions please don't hesitate to contact me,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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