Anti-LIM Kinase 1抗体[OTI3G3] (ab119084)
Key features and details
- Mouse monoclonal [OTI3G3] to LIM Kinase 1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG2b
概述
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产品名称
Anti-LIM Kinase 1抗体[OTI3G3]
参阅全部 LIM Kinase 1 一抗 -
描述
小鼠单克隆抗体[OTI3G3] to LIM Kinase 1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human LIM Kinase 1. Produced in HEK-293T cells. NP_002305.
Database link: P53667 -
阳性对照
- WB: HEK-293T cells transfected with LIM Kinase 1. ICC/IF: COS-7 cells transiently transfected with LIM Kinase 1. IHC-P: Human endometrium adenocarcinoma and lymphoma tissue.
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常规说明
The clone number has been updated from 3G3 to OTI3G3, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI3G3 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119084于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 73 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/2000. Predicted molecular weight: 73 kDa. |
ICC/IF
1/100. |
IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. -
组织特异性
Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. -
疾病相关
Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
细胞定位
Cytoplasm. Cell projection > growth cone. - Information by UniProt
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数据库链接
- Entrez Gene: 3984 Human
- Omim: 601329 Human
- SwissProt: P53667 Human
- Unigene: 647035 Human
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别名
- EC 2.7.1.37 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
see all
图片
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All lanes : Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected the pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1
Lysates/proteins at 5 µg per lane.
Predicted band size: 73 kDa -
Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human endometrium adenocarcinoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lymphoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab119084 被引用在 3 文献中.
- Ravindran S et al. BDNF Induced Translation of Limk1 in Developing Neurons Regulates Dendrite Growth by Fine-Tuning Cofilin1 Activity. Front Mol Neurosci 12:64 (2019). PubMed: 30949027
- Yao J et al. miR-133b regulates proliferation and apoptosis in high-glucose-induced human retinal endothelial cells by targeting ras homolog family member A. Int J Mol Med 42:839-850 (2018). PubMed: 29786744
- Yu L et al. c-Maf inducing protein inhibits cofilin-1 activity and alters podocyte cytoskeleton organization. Mol Med Rep 16:4955-4963 (2017). PubMed: 28791377