Anti-LIM Kinase 1抗体[OTI3G3] (ab119084)


  • 产品名称Anti-LIM Kinase 1抗体[OTI3G3]
    参阅全部 LIM Kinase 1 一抗
  • 描述
    小鼠单克隆抗体[OTI3G3] to LIM Kinase 1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein of Human LIM Kinase 1 produced in HEK293T cells (NP_002305) .

  • 阳性对照
    • HEK293T cells transfected with LIM Kinase 1, COS7 cells transiently transfected with LIM Kinase 1
  • 常规说明

    Concentration 0.5~1.0 mg/ml (Lot Dependent)

    The clone number has been updated from 3G3 to OTI3G3, both clone numbers name the same clone. 



Our Abpromise guarantee covers the use of ab119084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100.


  • 功能Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • 组织特异性Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • 疾病相关Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • 细胞定位Cytoplasm. Cell projection > growth cone.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC antibody
    • LIM domain containing protein kinase antibody
    • LIM domain kinase 1 antibody
    • LIM kinase antibody
    • LIM motif containing protein kinase antibody
    • LIMK 1 antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 antibody [OTI3G3] 图像

  • All lanes : Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected the pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 73 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC218058)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.

Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)参考文献

ab119084 has not yet been referenced specifically in any publications.

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