使用敲除细胞株进行验证RabMAb

Anti-LIM Kinase 1抗体[EPR912] (ab108507)

概述

  • 产品名称Anti-LIM Kinase 1抗体[EPR912]
    参阅全部 LIM Kinase 1 一抗
  • 描述
    兔单克隆抗体[EPR912] to LIM Kinase 1
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminal region of Human LIM Kinase 1.

  • 阳性对照
    • U-87 MG, SH SY5Y and Ramos cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

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应用

Our Abpromise guarantee covers the use of ab108507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • 组织特异性Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • 疾病相关Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • 细胞定位Cytoplasm. Cell projection > growth cone.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Anti-LIM Kinase 1 antibody [EPR912] 图像



    • Predicted band size : 73 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: LIMK1 knockout HAP1 cell lysate (40 µg)
      Lane 3: PC12 cell lysate (20 µg)
      Lane 4: NIH3T3 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108507 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108507 was shown to specifically react with LIM Kinase 1 when LIM Kinase 1 knockout samples were used. Wild-type and LIM Kinase 1 knockout samples were subjected to SDS-PAGE. Ab108507 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 73 kDa

    Anti-LIM Kinase 1 antibody [EPR912] (ab108507)参考文献

    ab108507 has not yet been referenced specifically in any publications.

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