Anti-LIM Kinase 1抗体(ab55414)

概述

  • 产品名称Anti-LIM Kinase 1抗体
    参阅全部 LIM Kinase 1 一抗
  • 描述
    小鼠单克隆抗体to LIM Kinase 1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ADPDYLPRTM DFGLNVRGFL DRYCPPNCPP SFFPITVRCC DLDPEKRPSF VKLEHWLETL RMHLAGHLPL GPQLEQLDRG FWETYRRGES GLPAHPEVPD , corresponding to amino acids 548-648 of Human LIM Kinase 1

性能

应用

Our Abpromise guarantee covers the use of ab55414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • 组织特异性Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • 疾病相关Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • 细胞定位Cytoplasm. Cell projection > growth cone.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Anti-LIM Kinase 1 antibody 图像

    • LIM Kinase 1 antibody (ab55414) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.


    • Predicted band size : 73 kDa
      LIM Kinase 1 antibody (ab55414) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

    Anti-LIM Kinase 1 antibody (ab55414)参考文献

    ab55414 has not yet been referenced specifically in any publications.

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