Anti-LIM Kinase 1抗体(ab10561)

概述

性能

应用

Our Abpromise guarantee covers the use of ab10561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).

靶标

  • 功能Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • 组织特异性Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • 疾病相关Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • 细胞定位Cytoplasm. Cell projection > growth cone.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.1.37 antibody
    • LIM domain containing protein kinase antibody
    • LIM domain kinase 1 antibody
    • LIM motif containing protein kinase antibody
    • LIMK 1 antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 antibody 图像



  • Predicted band size : 73 kDa


    Rabbit polyclonal to LIM kinase (1/500) - ab10561. 18 ug of mouse brain lysate loaded. Expected mol weight : 73 kD. Bands observed at 75 kD which we believe represents LIM kinase (doublet - may indicate modification such as phosphorylation) and 25 kD (we do not know the identity of this band). Secondary ab - Goat polyclonal to rabbit IgG (HRP) preabsorbed

Anti-LIM Kinase 1 antibody (ab10561)参考文献

ab10561 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab10561.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"