Anti-LIM kinase 1 + 2抗体(ab51200)

概述

  • 产品名称Anti-LIM kinase 1 + 2抗体
  • 描述
    兔多克隆抗体to LIM kinase 1 + 2
  • 特异性Detects endogenous levels of total LIMK1 and LIMK2 proteins. Ab51200 is produced using a phospho peptide immunogen. The phospho-specific antibody is removed by affinity column and pan antibody was purified by non-phospho-peptide columns. This antibody will therefore react with both phosphorylated and non phosphorylated forms of LIMK1 and LIMK2 proteins.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized non-phosphopeptide derived from human LIMK1 and LIMK2 around the phosphorylation site of threonine 508/505 (R-Y-TP-V-V).

  • 阳性对照
    • Human breast carcinoma tissue Extracts from cos7 cells

性能

应用

Our Abpromise guarantee covers the use of ab51200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 72 , 73 kDa).

靶标

  • 相关性LIMK 1 & 2 likely regulate aspects of the cytoskeleton, through control of the organization of actin filaments. They can phosphorylate an actin-binding protein, cofilin which binds to actin monomers and polymers and promotes the disassembly of actin filament.The phosphorylation of cofilin via LIMK inactivates this potential. LIMK1 is highly active in the brain and spinal chord, where it is believed to be involved in the development of nerve cells whilst LIMK2 is ubiquitously expressed in many adult tissues. LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition). These parts of the brain are important for visualizing an object as a set of parts and performing tasks such as writing, drawing, constructing models, and assembling puzzles. LIMK1 is specifically stimulated by Rac, one of the Rho family proteins, while LIMK2 activity is activated under the control of other Rho family members, Rho and Cdc42, suggesting that two distinct pathways exist in the Rho family driven actin cytoskeleton dynamics.
  • 细胞定位LIMK1 Cytoplasmic LIMK2 Cytoplasmic and Nuclear. Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • 数据库链接
  • 别名
    • LIM domain kinase 1 antibody
    • LIM domain kinase 2 antibody
    • LIMK1 antibody
    • LIMK2 antibody

Anti-LIM kinase 1 + 2 antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab51200 LIMK1/2 antibody.
  • All lanes : Anti-LIM kinase 1 + 2 antibody (ab51200)

    Lane 1 : Extracts from cos7 cells
    Lane 2 : Extracts from cos7 cells with Immunizing peptide


    Predicted band size : 72 , 73 kDa
    Observed band size : ~70 kDa (why is the actual band size different from the predicted?)

Anti-LIM kinase 1 + 2 antibody (ab51200)参考文献

ab51200 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - other (Post synaptic density enriched fractions of mouse)
Gel Running Conditions Reduced Denaturing (4-20% acrylamide gel)
Loading amount 15 µg
Treatment Bicuculline Methiodide (5µM)+ 4-Aminopyridine(40mM) and/or Beta-Amyloïd oligomers (100nM)
Specification Post synaptic density enriched fractions of mouse
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Mr. Marc Dollmeyer

Verified customer

提交于 Aug 03 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"