Anti-LIFR抗体(ab101228)

概述

  • 产品名称Anti-LIFR抗体
    参阅全部 LIFR 一抗
  • 描述
    兔多克隆抗体to LIFR
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Human, Pig, Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse LIFR.

  • 阳性对照
    • This antibody gave a positive signal in Mouse Placenta tissue lysate and in mouse placenta FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab101228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).

靶标

  • 功能Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
  • 疾病相关Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS) [MIM:601559]; also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue.
    Note=A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 6 fibronectin type-III domains.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD118 antibody
    • CD118 antigen antibody
    • FLJ98106 antibody
    • FLJ99923 antibody
    • Leukemia inhibitory factor receptor alpha antibody
    • Leukemia inhibitory factor receptor antibody
    • LIF R antibody
    • LIF receptor antibody
    • LIF-R antibody
    • Lifr antibody
    • LIFR_HUMAN antibody
    • SJS2 antibody
    • STWS antibody
    • SWS antibody
    see all

Anti-LIFR antibody 图像

  • Anti-LIFR antibody (ab101228) at 1 µg/ml + Mouse Placenta Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 122 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds
  • IHC image of LIFR staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101228, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-LIFR antibody (ab101228)参考文献

ab101228 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Colon
Blocking step UV inhibitor - Ventana as blocking agent for 4 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative Formaldehyde
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提交于 Jul 05 2016

Ich verstehe Ihre Bedenken.

Es tut mir leid, dass ich Ihnen in diesem Fall nicht mehr helfen konnte.

Ich wünsche Ihnen alles Gute bei der Suche und viel Erfolg mit Ihrem Experiment.

Sorry for the delay in getting back to you.

I now have some further information to share with you in regards to the antibody ab89792. This antibody is a mouse monoclonal which has been raised against a recombinant fragment of the human LIFR p...

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