快线抗体 仍未被充份测试和鉴定, 不在质保范围内, 具体请参见 使用条款及细节

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Lhx8

    该产品是 快线抗体,通过亲和纯化得到。对抗原肽有高灵敏度及特异性(ELISA测试)。 Read the terms of use »

  • 宿主
    Rabbit
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Lhx8.

    (Peptide available as ab45116.)

  • 阳性对照
    • This antibody gave a positive signal in the following Tissue Lysates: Mouse Liver Rat Liver Mouse Brain (data not shown)

性能

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.

靶标

图片

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-Lhx8 antibody (ab41519) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 43 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Shi J  et al. Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro. In Vitro Cell Dev Biol Anim 48:603-9 (2012). IHC . Read more (PubMed: 23150137) »
  • White YA  et al. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med 18:413-21 (2012). Read more (PubMed: 22366948) »

See all 2 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Embryonic day 19.5 brain tissue)
Permeabilization
No
Specification
Embryonic day 19.5 brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 12 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (neuroblastoma)
Loading amount
300000 cells
Specification
neuroblastoma
Gel Running Conditions
Non-reduced Denaturing (12% Bis-Tris)
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: RT°C
Username

Ann-Christin Schmädicke

Verified customer

提交于 Jan 17 2012

Vielen Dank für Ihre Email und den Abreview über ab41519. Diese Informationen sind sehr wichtig für andere Forscher und wir schätzen Ihr Feedback sehr. Der Gutscheincode wird gültig sobald ein Abreview eingereicht ist. Ihr Gutscheincode ist somit ab ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册