概述

  • 产品名称Anti-LHX6抗体
    参阅全部 LHX6 一抗
  • 描述
    兔多克隆抗体to LHX6
  • 特异性ab22885 recognises LHX6
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Dog不与反应: Mouse
  • 免疫原

    Synthetic peptide: RRVIQVWFQN CRARHKKHTP QHPVPPSGAP PSRLPSALSD DIHYTPFSSP, corresponding to a region within C terminal amino acids 289-338 of Human LHX6

  • 阳性对照
    • HepG2 cell lysate and human liver tissue.

性能

应用

Our Abpromise guarantee covers the use of ab22885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Probable transcription factor required for the expression of a subset of genes involved in interneurons migration and development. Functions in the specification of cortical interneuron subtypes and in the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LHX 6 antibody
    • LHX 6.1 antibody
    • LHX6 antibody
    • LHX6.1 antibody
    • LHX6_HUMAN antibody
    • LIM homeobox 6 antibody
    • LIM homeobox protein 6 antibody
    • LIM homeodomain protein 6.1 antibody
    • LIM/homeobox protein Lhx6 antibody
    • LIM/homeobox protein Lhx6.1 antibody
    • MGC119542 antibody
    • MGC119544 antibody
    • MGC119545 antibody
    • OTTHUMP00000064113 antibody
    • OTTHUMP00000064114 antibody
    see all

Anti-LHX6 antibody 图像

  • Immunohistochemical analysis of LHX6 expression in paraffin embedded human liver tissue. ab22885 was used at 4 ug/ml. Arrows indicate positively labelled hepatocytes.
  • Lane 1 : Anti-LHX6 antibody (ab22885) at 5 µg/ml
    Lane 2 : Anti-LHX6 antibody (ab22885) at 2.5 µg/ml
    Lane 3 : Anti-LHX6 antibody (ab22885) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared from human HepG2 cells
    Lane 2 : Cell lysate prepared from human HepG2 cells
    Lane 3 : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-LHX6 antibody (ab22885)参考文献

This product has been referenced in:
  • García-Moreno F  et al. A neuronal migratory pathway crossing from diencephalon to telencephalon populates amygdala nuclei. Nat Neurosci 13:680-9 (2010). IHC-P ; Mouse . Read more (PubMed: 20495559) »
  • Jakovcevski I  et al. Multiple Origins of Human Neocortical Interneurons Are Supported by Distinct Expression of Transcription Factors. Cereb Cortex : (2010). IHC-Fr ; Human . Read more (PubMed: 21139075) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human fibroblast and stem cells)
Loading amount 50 µg
Specification human fibroblast and stem cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2012

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Thank you also for the image that you have provided me with, it is most useful in understanding the difficulties that you have experienced. As...

Read More

Thank you for your enquiry. I am sorry to hear you are having a problem with ab22885 (LHX6 antibody). To improve the detection of LHX6, I would like to suggest the following modifications to your protocol: 1) As LHX6 is a nuclear protein, use RI...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain, fixed in acrolein)
Specification brain, fixed in acrolein
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 6%
Username

Miss. Nina Hasen

Verified customer

提交于 Jun 01 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"