概述

  • 产品名称Anti-LHX5抗体
    参阅全部 LHX5 一抗
  • 描述
    小鼠单克隆抗体to LHX5
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
  • 免疫原

    Recombinant fragment: CTDRSLSPDL QDALQDDPKE TDNSTSSDKE TANNENEEQN SGTKRRGPRT TIKAKQLETL KAAFAATPKP TRHIREQLAQ ETGLNMRVIQ VWFQNRRSKE, corresponding to amino acids 136-235 of Human LHX5 (NP_071758) with a proprietary tag.

  • 阳性对照
    • Partial recombinant tagged LHX5 protein. 293, MES-SA/DX5, Y-79, and Jurkat cell lysates. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged LHX5 is approximately 3ng/ml when used as a capture antibody.
ICC/IF Use a concentration of 10 µg/ml.

靶标

Anti-LHX5 antibody 图像

  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + Partial recombinant tagged LHX5 (Immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + 293 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + MES-SA/Dx5 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + Y-79 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • ab88185, at 10 µg/ml, staining LHX5 in permeabilised, paraformaldehyde fixed HeLa cells by immunofluorescence.

Anti-LHX5 antibody (ab88185)参考文献

ab88185 has not yet been referenced specifically in any publications.

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