Anti-LHX3抗体(ab26025)

概述

  • 产品名称Anti-LHX3抗体
    参阅全部 LHX3 一抗
  • 描述
    兔多克隆抗体to LHX3
  • 特异性ab26025 recognises LHX3.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: EKRLKKDAGR QRWGQYFRNM KRSRGGSKSD KDSVQEGQDS DAEVSFPDEP, corresponding to an sequence within internal sequence amino acids 217-266 of Human LHX3

  • 阳性对照
    • Jurkat cell lysate, human lung tissue, human heart tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab26025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
  • 疾病相关Defects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 结构域The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CPHD 3 antibody
    • CPHD3 antibody
    • DKFZp762A2013 antibody
    • LHX 3 antibody
    • LHX3 antibody
    • LHX3_HUMAN antibody
    • LIM 3 antibody
    • LIM homeobox 3 antibody
    • LIM homeobox gene 3 antibody
    • LIM homeobox protein 3 antibody
    • LIM/homeobox protein Lhx3 antibody
    • LIM/homeodomain protein LHX3 antibody
    • Lim3 antibody
    • M2 LHX3 antibody
    • mLim-3 antibody
    • mLIM3 antibody
    • P LIM antibody
    see all

Anti-LHX3 antibody 图像

  • Anti-LHX3 antibody (ab26025) at 1.25 µg/ml

    Predicted band size : 44 kDa
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded lung tissue. Cells with positive label (as indicated by the arrows) are alveclar cells. Magnification 400X.
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded heart tissue. Cells with positive label (as indicated by the arrows) are myocardial cells. Magnification 400X.

Anti-LHX3 antibody (ab26025)参考文献

ab26025 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). I hop...

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