概述

  • 产品名称Anti-LexA DNA Binding Region抗体
    参阅全部 LexA DNA Binding Region 一抗
  • 描述
    兔多克隆抗体to LexA DNA Binding Region
  • 特异性This antibody recognizes E. coli LexA and LexA DBD-tagged fusion proteins.
  • 经测试应用适用于: WB, IPmore details
  • 免疫原

    Full length protein corresponding to the DNA binding domain (DBD, amino acids 1-222) of E. coli LexA.

性能

应用

Our Abpromise guarantee covers the use of ab50953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). This product has been tested by Western blotting using a LexA-Rb fusion and a LexA-PP2A, beta subunit fusion expressed in budding yeast. The observed molecular weight of a fusion protein will be increased by approximately 24 kDa due to the addition of the LexA DNA binding domain.
IP 1/1.

靶标

  • 相关性The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
  • 细胞定位Nuclear
  • 别名
    • ExrA antibody
    • Lex A antibody
    • LexA antibody
    • LexA repressor antibody
    • Spr antibody
    • Tsl antibody
    • UmuA antibody
    see all

Anti-LexA DNA Binding Region antibody 图像

  • All lanes : Anti-LexA DNA Binding Region antibody (ab50953) at 1/100000 dilution

    Lane 1 : Crude yeast protein extract without a LexA fusion protein
    Lane 2 : Crude yeast protein extract expressing a LexA-Retinoblastoma (Rb) fusion protein

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 49 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-LexA DNA Binding Region antibody (ab50953)参考文献

This product has been referenced in:
  • Odsbu I & Skarstad K DNA compaction in the early part of the SOS response is dependent on RecN and RecA. Microbiology 160:872-82 (2014). WB ; Escherichia coli . Read more (PubMed: 24615185) »
  • Erental A  et al. Apoptosis-like death, an extreme SOS response in Escherichia coli. MBio 5:e01426-14 (2014). WB ; Escherichia coli . Read more (PubMed: 25028428) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab50953.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"