Anti-Leukotriene B4 Receptor抗体(ab82851)


  • 产品名称
    Anti-Leukotriene B4 Receptor抗体
    参阅全部 Leukotriene B4 Receptor 一抗
  • 描述
    兔多克隆抗体to Leukotriene B4 Receptor
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Leukotriene B4 Receptor.

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Heart; Thyroid; Thymus.



Our Abpromise guarantee covers the use of ab82851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa).


  • 功能
    Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
  • 组织特异性
    Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰
    Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BLT antibody
    • BLT1 antibody
    • BLTR antibody
    • Chemoattractant receptor-like 1 antibody
    • Chemokine receptor like 1 antibody
    • CMKRL1 antibody
    • G protein coupled receptor 16 antibody
    • G-protein coupled receptor 16 antibody
    • GPR16 antibody
    • Leukotriene B4 G Protein coupled receptor antibody
    • Leukotriene B4 receptor 1 antibody
    • Leukotriene B4 receptor antibody
    • LT4R1_HUMAN antibody
    • LTB4 R 1 antibody
    • LTB4-R 1 antibody
    • LTB4-R1 antibody
    • LTB4R 1 antibody
    • Ltb4r antibody
    • LTB4R1 antibody
    • LTBR1 antibody
    • P2RY7 antibody
    • P2Y purinoceptor 7 antibody
    • P2Y7 antibody
    • Purinergic receptor P2Y G protein coupled 7 antibody
    see all

Anti-Leukotriene B4 Receptor antibody 图像

  • All lanes : Anti-Leukotriene B4 Receptor antibody (ab82851) at 1 µg/ml

    Lane 1 : Human thyroid normal tissue lysate - total protein (ab29636)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 20 µg per lane.

    Lanes 1 - 2 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,62 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab82851 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82851, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

Anti-Leukotriene B4 Receptor antibody (ab82851)参考文献

ab82851 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting your review for ab82851 for western blotting of rat samples. I have compared the immunogen sequence use to produce ab82851 with the that of the rat sequence. Unfortunately they are quite divergent so I would not expect this ant...

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Rat Tissue lysate - whole (rat ischemic liver)
Loading amount
10 µg
rat ischemic liver
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Jung nam hee

Verified customer

提交于 Feb 15 2012

I am very pleased to hear you would like to test ab82851 in mouse or rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse or rat and include this code in the “Additio...

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