Anti-Leukotriene B4 Receptor 2抗体(ab84600)

概述

  • 产品名称Anti-Leukotriene B4 Receptor 2抗体
    参阅全部 Leukotriene B4 Receptor 2 一抗
  • 描述
    兔多克隆抗体to Leukotriene B4 Receptor 2
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Leukotriene B4 Receptor 2.

    (Peptide available as ab94553.)

  • 阳性对照
    • This antibody gave a positive signal in Human brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

靶标

Anti-Leukotriene B4 Receptor 2 antibody 图像

  • Anti-Leukotriene B4 Receptor 2 antibody (ab84600) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 15 minutes
  • ICC/IF image of ab84600 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84600, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Leukotriene B4 Receptor 2 antibody (ab84600)参考文献

ab84600 has not yet been referenced specifically in any publications.

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