Anti-Leukotriene A4 hydrolase抗体(ab23677)

概述

  • 产品名称Anti-Leukotriene A4 hydrolase抗体
    参阅全部 Leukotriene A4 hydrolase 一抗
  • 描述
    兔多克隆抗体to Leukotriene A4 hydrolase
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Cow, Human
    预测可用于: Rat, Guinea pig
  • 免疫原

    Recombinant full length protein (Human)

性能

应用

Our Abpromise guarantee covers the use of ab23677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa.

靶标

Anti-Leukotriene A4 hydrolase antibody 图像

  • All lanes : Anti-Leukotriene A4 hydrolase antibody (ab23677) at 2 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

Anti-Leukotriene A4 hydrolase antibody (ab23677)参考文献

This product has been referenced in:
  • Korzekwa AJ  et al. Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites. Reprod Biol Endocrinol 9:27 (2011). WB ; Cow . Read more (PubMed: 21349168) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Peripheral blood mononuclear cells)
Loading amount 20 µg
Specification Peripheral blood mononuclear cells
Gel Running Conditions Non-reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Mar 31 2009

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"