ab84626 detects isoform 3.
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (KNYESKKYSD PSQPGNTVLH PGTRLIQKLH TSTCWLQEVP GKPQLEQATK) of human LETM2 (NP_653253).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Contains 1 LETM1 domain.
Mitochondrion inner membrane.
Information by UniProt
- LETM1 and EF hand domain containing protein 2 antibody
- LETM1 and EF-hand domain-containing protein 2 antibody
- LETM1 domain containing protein LETM2 mitochondrial antibody
Western blot - LETM2 antibody (ab84626)
Anti-LETM2 antibody (ab84626) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
45 kDaObserved band size :
40 kDa (why is the actual band size different from the predicted?
)Additional bands at :
60 kDa (possible isoform),>90 kDa. We are unsure as to the identity of these extra bands.
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