Anti-Leptin Receptor抗体[LPR-02] (ab2143)

概述

  • 产品名称Anti-Leptin Receptor抗体[LPR-02]
    参阅全部 Leptin Receptor 一抗
  • 描述
    小鼠单克隆抗体[LPR-02] to Leptin Receptor
  • 特异性Mouse antibody reacts with leptin receptor-Fc in indirect ELISA and does not react with human serum immunoglobulins.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ELISA, Indirect ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-839 of Human Leptin Receptor fused to the Fc region of human IgG (with IIEGR added at the amino terminus and 6 histidine residues added at the carboxy terminus). Will detect all isoforms of the leptin receptor

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.

性能

应用

Our Abpromise guarantee covers the use of ab2143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IHC-P 1/10 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.
Indirect ELISA Use at an assay dependent concentration.

靶标

  • 功能Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
  • 组织特异性Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like (immunoglobulin-like) domain.
  • 结构域The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 翻译后修饰On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 295 antibody
    • CD295 antibody
    • CD295 antigen antibody
    • Db antibody
    • Fa antibody
    • HuB219 antibody
    • LEP R antibody
    • LEP-R antibody
    • LEPR antibody
    • LEPR_HUMAN antibody
    • LEPRD antibody
    • Leptin receptor antibody
    • Leptin receptor fatty antibody
    • Leptin receptor gene related protein antibody
    • Leptin receptor precursor antibody
    • Leptin receptor precursor antibody
    • OB R gene related protein antibody
    • OB receptor antibody
    • OB-R antibody
    • OB-RGRP antibody
    • obl antibody
    • Obr antibody
    see all

Anti-Leptin Receptor antibody [LPR-02] 图像

  • ICC/IF image of ab2143 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2143 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Leptin Receptor antibody [LPR-02] (ab2143)参考文献

ab2143 has not yet been referenced specifically in any publications.

Product Wall

These two monoclonals are different clones - to our knowledge they have the same activity

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"