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As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 21151569|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837). Corresponding to the long and short forms (respectively) of leptin receptor OB-R from mouse brain extract. Two smaller bands of approximately 31 and 35 kDa are also detected, which cannot be competed out with immunogenic peptide. Block with 5% milk in TBST at RT. The native leptin receptor is a homodimer held together with disulfide bonds. On a reducing SDS-PAGE, bands may appear as approximately 60 kDa subunits. Further variations in size is possible and is due to glycosylation.|
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Blocking agent: 5% Milk for 1 hour at 23°C.
Western blot of both short and long forms of Leptin Receptor on mouse lung tissue extract using ab5593.
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