Anti-Leptin Receptor抗体(ab50424)


  • 产品名称Anti-Leptin Receptor抗体
    参阅全部 Leptin Receptor 一抗
  • 描述
    山羊多克隆抗体to Leptin Receptor
  • 特异性This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1) of Leptin Receptor.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide:


    , corresponding to internal sequence amino acids 829-841 of Human Leptin Receptor

  • 阳性对照
    • Human Brain lysate.



Our Abpromise guarantee covers the use of ab50424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa).
ELISA Use at an assay dependent concentration.


  • 功能Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
  • 组织特异性Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like (immunoglobulin-like) domain.
  • 结构域The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 翻译后修饰On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 295 antibody
    • CD295 antibody
    • CD295 antigen antibody
    • Db antibody
    • Fa antibody
    • HuB219 antibody
    • LEP R antibody
    • LEP-R antibody
    • LEPR antibody
    • LEPR_HUMAN antibody
    • LEPRD antibody
    • Leptin receptor antibody
    • Leptin receptor fatty antibody
    • Leptin receptor gene related protein antibody
    • Leptin receptor precursor antibody
    • Leptin receptor precursor antibody
    • OB R gene related protein antibody
    • OB receptor antibody
    • OB-R antibody
    • OB-RGRP antibody
    • obl antibody
    • Obr antibody
    see all

Anti-Leptin Receptor antibody 图像

Anti-Leptin Receptor antibody (ab50424)参考文献

ab50424 has not yet been referenced specifically in any publications.

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We have a number of different antibodies directed against the human Leptin protein. The ideal choices for your experiment would depend on what application you are hoping to use. <...

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Thank you for your enquiry. I can confirm that the immunizing peptide is in all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1). NCBI calls them isoforms 1, 2, and 3 resp, but the alternative named isoforms are CRA_b, CRA_c and...

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