Anti-Leptin抗体(ab98090)

概述

  • 产品名称Anti-Leptin抗体
    参阅全部 Leptin 一抗
  • 描述
    兔多克隆抗体to Leptin
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Goat, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS) of Human Leptin (NP_000221).

  • 阳性对照
    • Human fetal small intestine lysate

性能

应用

Our Abpromise guarantee covers the use of ab98090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • 疾病相关Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性Belongs to the leptin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

Anti-Leptin antibody 图像

  • Anti-Leptin antibody (ab98090) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    Predicted band size : 19 kDa
  • ab98090 staining Leptin in placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-Leptin antibody (ab98090)参考文献

ab98090 has not yet been referenced specifically in any publications.

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With regards to your technical questions:

1.Did you try to block the membrane in 5% BSA instead of 5% milk? Is it 1 hour incubation at RT?
We only use 5% milk for blocking the membrane. Either...

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