概述

  • 产品名称Anti-Leptin抗体
    参阅全部 Leptin 一抗
  • 描述
    兔多克隆抗体to Leptin
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Human, Chimpanzee, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Leptin.

  • 阳性对照
    • This antibody gave a positive signal in Rat Heart tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab117751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 18 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • 疾病相关Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性Belongs to the leptin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

Anti-Leptin antibody 图像

  • ICC/IF image of ab117751 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab117751 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Leptin antibody (ab117751) at 1 µg/ml + Heart (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa,49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    The band observed at 15 kDa could potentially be a cleaved form of Leptin due to the presence of a 21 amino acid signal peptide. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117751 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • IHC image of Leptin staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117751, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Leptin antibody (ab117751)参考文献

ab117751 has not yet been referenced specifically in any publications.

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