Anti-LEO1抗体- N-terminal (ab75721)

概述

  • 产品名称
    Anti-LEO1抗体- N-terminal
    参阅全部 LEO1 一抗
  • 描述
    兔多克隆抗体to LEO1 - N-terminal
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the N-terminal region of human LEO1.

  • 阳性对照
    • HL60 cell line lysate. 293 cell lysate transiently transfected with the LEO1 gene.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab75721 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/250. Predicted molecular weight: 75 kDa.
ELISA 1/1000.

靶标

  • 功能
    The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.
  • 组织特异性
    Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
  • 序列相似性
    Belongs to the LEO1 family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gm185 antibody
    • LEO1 antibody
    • LEO1_HUMAN antibody
    • LOC123439 antibody
    • Paf1/RNA polymerase II complex component antibody
    • Replicative senescence down regulated leo1 like protein antibody
    • Replicative senescence down-regulated leo1-like protein antibody
    • Replicative senescence downregulated leo1 like antibody
    • RNA polymerase associated protein LEO1 antibody
    • RNA polymerase-associated protein LEO1 antibody
    • Senescence downregulated leo1 like antibody
    see all

图片

  • Anti-LEO1 antibody - N-terminal (ab75721) at 1/50 dilution + HL60 cell line lysate at 35 µg

    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-LEO1 antibody - N-terminal (ab75721) at 1/100 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the LEO1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab75721 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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