重组Anti-Legumain抗体[EPR14718] - BSA and Azide free (ab232627)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14718] to Legumain - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Legumain抗体[EPR14718] - BSA and Azide free
参阅全部 Legumain 一抗 -
描述
兔单克隆抗体[EPR14718] to Legumain - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal kidney tissue lysate; JAR, HeLa and HEK-293 whole cell lysates.
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常规说明
ab232627 is the carrier-free version of ab183028.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14718 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232627于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
靶标
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功能
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
组织特异性
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
序列相似性
Belongs to the peptidase C13 family. -
翻译后修饰
Glycosylated. -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 5641 Human
- Entrez Gene: 19141 Mouse
- Entrez Gene: 63865 Rat
- Omim: 602620 Human
- SwissProt: Q99538 Human
- SwissProt: O89017 Mouse
- SwissProt: Q9R0J8 Rat
- Unigene: 726036 Human
see all -
别名
- AEP antibody
- Asparaginyl endopeptidase antibody
- cysteine 1 antibody
see all
图片
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All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : JAR (human placenta choriocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDa
Exposure time: 3 minutesActual band at: 36kDa, 46kDa, 56kDa.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183028).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab232627 尚未被引用在任何文献中。