重组Anti-Legumain抗体[EPR14718] (ab183028)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14718] to Legumain
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Legumain抗体[EPR14718]
参阅全部 Legumain 一抗 -
描述
兔单克隆抗体[EPR14718] to Legumain -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal kidney lysate; JAR, HeLa and 293 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14718 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183028于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 49 kDa. |
靶标
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功能
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
组织特异性
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
序列相似性
Belongs to the peptidase C13 family. -
翻译后修饰
Glycosylated. -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 5641 Human
- Entrez Gene: 19141 Mouse
- Entrez Gene: 63865 Rat
- Omim: 602620 Human
- SwissProt: Q99538 Human
- SwissProt: O89017 Mouse
- SwissProt: Q9R0J8 Rat
- Unigene: 726036 Human
see all -
别名
- AEP antibody
- Asparaginyl endopeptidase antibody
- cysteine 1 antibody
see all
图片
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All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : JAR (human placenta choriocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDa
Exposure time: 3 minutesActual band at: 36kDa, 46kDa, 56kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab183028 被引用在 4 文献中.
- Chen C et al. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI. Cell Death Dis 12:65 (2021). PubMed: 33431801
- Lian J et al. Legumain acts on neuroinflammatory to affect CUS-induced cognitive impairment. Behav Brain Res 376:112219 (2019). PubMed: 31509774
- Wang C et al. Lysophosphatidic acid induces neuronal cell death via activation of asparagine endopeptidase in cerebral ischemia-reperfusion injury. Exp Neurol 306:1-9 (2018). WB ; Rat . PubMed: 29673933
- Završnik J et al. Cystatin C deficiency suppresses tumor growth in a breast cancer model through decreased proliferation of tumor cells. Oncotarget 8:73793-73809 (2017). PubMed: 29088746