Anti-Legumain抗体(ab125286)

概述

  • 产品名称Anti-Legumain抗体
    参阅全部 Legumain 一抗
  • 描述
    兔多克隆抗体to Legumain
  • 经测试应用适用于: WB, IP, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 100-190 of Human Legumain. This sequence is identical in Mouse and Rat.

  • 阳性对照
    • HT29, HeLa, and Mouse Kidney lysates; Human ovary carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab125286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • 组织特异性Ubiquitous. Particularly abundant in kidney, heart and placenta.
  • 序列相似性Belongs to the peptidase C13 family.
  • 翻译后修饰Glycosylated.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AEP antibody
    • Asparaginyl endopeptidase antibody
    • cysteine 1 antibody
    • Cysteine protease 1 antibody
    • EC 3.4.22.34 antibody
    • Legumain antibody
    • LGMN antibody
    • LGMN_HUMAN antibody
    • LGMN1 antibody
    • Protease antibody
    • Protease cysteine 1 (legumain) antibody
    • Protease cysteine 1 antibody
    • PRSC1 antibody
    see all

Anti-Legumain antibody 图像

  • All lanes : Anti-Legumain antibody (ab125286) at 1/500 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Mouse Kidney lysate


    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab125286, at 1/200 dilution, staining Legumain in formalin-fixed, paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

Anti-Legumain antibody (ab125286)参考文献

ab125286 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Leech (Hirudo medicinalis) Tissue lysate - whole (Central Nervous System)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 50 µg
Specification Central Nervous System
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"