The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
See attached protocol.
Legionella pneumophila is a thin, pleomorphic, flagellated Gram-negative bacterium of the genus Legionella and is the causative agent of legionellosis or Legionnaires' disease.
L. pneumophila is non-acid-fast, non-sporulating and morphologically a non-capsulated rod-like bacteria. Aerobic and unable to hydrolyse gelatin or produce urease, they are also non-fermentative. L. pneumophila is neither pigmented nor does it autofluoresce. It is oxidase- and catalase-positive and produces beta-lactamase.
While L. pneumophila is categorized as a Gram-negative organism, it stains poorly due to its unique lipopolysaccharide-content in the outer leaflet of the outer cell membrane. At least 35 different serovars of L. pneumophila have been described.