Anti-Lefty抗体- C-terminal (ab71268)

概述

  • 产品名称Anti-Lefty抗体- C-terminal
    参阅全部 Lefty 一抗
  • 描述
    兔多克隆抗体to Lefty - C-terminal
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 阳性对照
    • HL60 cell line lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明ab71268 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab71268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/50.
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

靶标

  • 相关性During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • AI450052 antibody
    • Ebaf antibody
    • Left right determination factor 1 antibody
    • Left right determination factor antibody
    • Left right determination factor B antibody
    • left-right determination factor antibody
    • LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1 antibody
    • left-right determination, factor B antibody
    • LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B antibody
    • LEFTB antibody
    • LEFTY 1 antibody
    • Lefty antibody
    • lefty-1 antibody
    • LEFTY1 antibody
    • LEFTY1, MOUSE, HOMOLOG OF antibody
    • LEFTYB antibody
    • OTTHUMP00000035572 antibody
    • Protein lefty-1 antibody
    • Protein lefty-B antibody
    • RGD1561867 antibody
    • Stimulated by retinoic acid gene 3 protein antibody
    • STRA3 antibody
    • TGF beta 4 antibody
    • TGF-beta-4 antibody
    • Tgfb4 antibody
    • Transforming growth factor beta 4 antibody
    • Transforming growth factor beta-4 antibody
    see all

Anti-Lefty antibody - C-terminal 图像

  • Anti-Lefty antibody - C-terminal (ab71268) at 1/60 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ab71268 at 1/50 dilution staining DOK6 in human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

Anti-Lefty antibody - C-terminal (ab71268)参考文献

ab71268 has not yet been referenced specifically in any publications.

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