概述

  • 产品名称Anti-Lefty抗体
    参阅全部 Lefty 一抗
  • 描述
    兔多克隆抗体to Lefty
  • 特异性This antibody has been tested against recombinant protein only (human LeftyA and mouse Lefty1). I is expected to cross react with Human LeftyB as there is 100% homology of the immunogen with Human LeftyB.
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Lefty.

    (Peptide available as ab25701.)

  • 阳性对照
    • recombinant Lefty1/ LeftyA

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
IHC-P
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Detects a bands of approximately 44, 38 and 34 kDa (predicted molecular weight: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
    • 细胞定位Secreted
    • 数据库链接
    • 别名
      • AI450052 antibody
      • Ebaf antibody
      • Left right determination factor 1 antibody
      • Left right determination factor antibody
      • Left right determination factor B antibody
      • left-right determination factor antibody
      • LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1 antibody
      • left-right determination, factor B antibody
      • LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B antibody
      • LEFTB antibody
      • LEFTY 1 antibody
      • Lefty antibody
      • lefty-1 antibody
      • LEFTY1 antibody
      • LEFTY1, MOUSE, HOMOLOG OF antibody
      • LEFTYB antibody
      • OTTHUMP00000035572 antibody
      • Protein lefty-1 antibody
      • Protein lefty-B antibody
      • RGD1561867 antibody
      • Stimulated by retinoic acid gene 3 protein antibody
      • STRA3 antibody
      • TGF beta 4 antibody
      • TGF-beta-4 antibody
      • Tgfb4 antibody
      • Transforming growth factor beta 4 antibody
      • Transforming growth factor beta-4 antibody
      see all

    Anti-Lefty antibody 图像

    • ICC/IF image of ab22569 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • All lanes : Anti-Lefty antibody (ab22569) at 1 µg/ml

      Lane 1 : Mouse Lefty1 Recombinant Protein
      Lane 2 : Human LeftyA Recombinant Protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

      Predicted band size : 41 kDa
      Observed band size : 34,38,44 kDa (why is the actual band size different from the predicted?)

    Anti-Lefty antibody (ab22569)参考文献

    This product has been referenced in:
    • Sun G  et al. Lefty inhibits glioma growth by suppressing Nodal-activated Smad and ERK1/2 pathways. J Neurol Sci 347:137-42 (2014). Read more (PubMed: 25304053) »
    • Barroso-delJesus A  et al. The Nodal inhibitor Lefty is negatively modulated by the microRNA miR-302 in human embryonic stem cells. FASEB J 25:1497-508 (2011). WB, IHC (PFA fixed) ; Human . Read more (PubMed: 21266536) »

    See all 2 Publications for this product

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (E6.5 embryo in decidua)
    Specification E6.5 embryo in decidua
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Username

    Dr. jesse mager

    Verified customer

    提交于 Feb 13 2009

    Thank you for your enquiry regarding ab22569. This antibody will recognise human lefty1 and left2 according to a BLAST search with the immunogen sequence though it has only been tested against recombinant Lefty1. Regarding its cross reactivity to mou...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"