Anti-LEF1 (phospho S42)抗体(ab74067)


  • 产品名称Anti-LEF1 (phospho S42)抗体
    参阅全部 LEF1 一抗
  • 描述
    兔多克隆抗体to LEF1 (phospho S42)
  • 特异性ab74067 detects endogenous levels of LEF1 only when phosphorylated at serine 42.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of serine 42 (E-I-SP-H-P).

  • 阳性对照
    • 293 cell extracts treated with paclitaxel (1uM, 24hours).



Our Abpromise guarantee covers the use of ab74067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/20000.


  • 功能Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • 组织特异性Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • 序列相似性Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • 结构域Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • 细胞定位Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Anti-LEF1 (phospho S42) antibody 图像

  • All lanes : Anti-LEF1 (phospho S42) antibody (ab74067) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with paclitaxel (1uM, 24hours)
    Lane 2 : 293 cell extracts treated with paclitaxel (1uM, 24hours) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

Anti-LEF1 (phospho S42) antibody (ab74067)参考文献

ab74067 has not yet been referenced specifically in any publications.

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