重组
RabMAb

Anti-LEF1抗体[EP2030Y] (ab53293)

概述

  • 产品名称
    Anti-LEF1抗体[EP2030Y]
    参阅全部 LEF1 一抗
  • 描述
    兔单克隆抗体[EP2030Y] to LEF1
  • 经测试应用
    适用于: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in human LEF1.

  • 阳性对照
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab53293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa).
Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
    • 组织特异性
      Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
    • 序列相似性
      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • 结构域
      Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
    • 细胞定位
      Nucleus. Found in nuclear bodies upon PIASG binding.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp586H0919 antibody
      • FLJ46390 antibody
      • LEF 1 antibody
      • LEF-1 antibody
      • Lef1 antibody
      • LEF1_HUMAN antibody
      • Lymphoid enhancer binding factor 1 antibody
      • Lymphoid enhancer-binding factor 1 antibody
      • T cell specific transcription factor 1 alpha antibody
      • T cell-specific transcription factor 1-alpha antibody
      • TCF 1 alpha antibody
      • TCF1 alpha antibody
      • TCF1-alpha antibody
      • TCF10 antibody
      • TCF1alpha antibody
      • TCF7L3 antibody
      • Transcription factor T cell specific 1 alpha antibody
      see all

    图片

    • ab53293 staining LEF1 in RAMOS (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.

    • Anti-LEF1 antibody [EP2030Y] (ab53293) at 1/1000 dilution + Jurkat whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 44 kDa
      Observed band size : 25-57 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking/Diluting buffer 5% NFDM/TBST

    • Ab53293 (1:50) staining human LEF1 in human colon adenocarcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Flow cytometric analysis of permeabilized Jurkat cells using ab53293 (red) or a rabbit IgG (negative) (green).

    文献

    This product has been referenced in:
    • Zhang X  et al. MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/ß-catenin pathway. Am J Cancer Res 6:2631-2640 (2016). WB ; Human . Read more (PubMed: 27904776) »
    • Shaikh LH  et al. LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal. J Clin Endocrinol Metab 100:E836-44 (2015). IHC ; Human . Read more (PubMed: 25915569) »

    See all 6 Publications for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (unerupted tooth buds)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citric Acid monobasic pH6
    Permeabilization
    No
    Specification
    unerupted tooth buds
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    10% Formalin
    Username

    Abcam user community

    Verified customer

    提交于 Apr 27 2017

    Thank you for your inquiry.
    The exact immunogen sequence is proprietary information and I unfortunately do not have access to it.
    But the lab confirmed that all isoforms should be recognized by ab53293 since all isoforms contain the immunogen...

    Read More

    Thank you for taking the time to contact us. I am sorry to hearthe customer has some concerns regarding the results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and...

    Read More

    Thank you for contacting Abcam.

    I am sorry that you are having problems with ab53293 in western blot. If you are looking for a positive control then I would recommend ab7899:

    http://www.abcam.com/Jurkat-Human-T-cell-lymphoblast-like...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (REH B cell)
    Specification
    REH B cell
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - saponin
    Username

    Abcam user community

    Verified customer

    提交于 Apr 01 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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