Anti-LEF1抗体(ab85052)
Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LEF1抗体
参阅全部 LEF1 一抗 -
描述
兔多克隆抗体to LEF1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
A synthetic peptide from intermediate residues of Human LEF1 protein, Swiss Prot Q9UJU2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 44 kDa).
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说明 |
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WB
1/200 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 44 kDa). |
靶标
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功能
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. -
组织特异性
Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. -
序列相似性
Belongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain. -
结构域
Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. -
细胞定位
Nucleus. Found in nuclear bodies upon PIASG binding. - Information by UniProt
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数据库链接
- Entrez Gene: 51176 Human
- Entrez Gene: 161452 Rat
- Omim: 153245 Human
- SwissProt: Q9UJU2 Human
- SwissProt: Q9QXN1 Rat
- Unigene: 726506 Human
- Unigene: 21926 Rat
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别名
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab85052 被引用在 6 文献中.
- Jiang C et al. NR4A1 promotes LEF1 expression in the pathogenesis of papillary thyroid cancer. Cell Death Discov 8:46 (2022). PubMed: 35110542
- Wang Q et al. Akt/mTOR integrate energy metabolism with Wnt signal to influence wound epithelium growth in Gekko Japonicus. Commun Biol 5:1018 (2022). PubMed: 36167813
- Aloysius A et al. The transcription factor Lef1 switches partners from ß-catenin to Smad3 during muscle stem cell quiescence. Sci Signal 11:N/A (2018). PubMed: 30042129
- Zhang T et al. Regulating osteogenesis and adipogenesis in adipose-derived stem cells by controlling underlying substrate stiffness. J Cell Physiol 233:3418-3428 (2018). PubMed: 28926111
- Jiang S et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep 14:1891-900 (2016). WB ; Human . PubMed: 27432616
- Meng Z et al. Tumorigenicity analysis of heterogeneous dental stem cells and its self-modification for chromosome instability. Cell Cycle 14:3396-407 (2015). PubMed: 26322910