概述

性能

应用

Our Abpromise guarantee covers the use of ab22884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • 组织特异性Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • 序列相似性Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • 结构域Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • 细胞定位Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Anti-LEF1 antibody 图像

  • Anti-LEF1 antibody (ab22884) at 2 µg/ml + Jurkat cell lysate with 5% skim milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Gel concentration is 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling LEF1 with ab22884 at 4µg/ml. Arows indicate the positively stained intestinal villus epithelial cells.

Anti-LEF1 antibody (ab22884)参考文献

This product has been referenced in:
  • Soma T  et al. Hair-inducing ability of human dermal papilla cells cultured under Wnt/ß-catenin signalling activation. Exp Dermatol 21:307-9 (2012). WB ; Human . Read more (PubMed: 22417309) »
  • Spee B  et al. Characterisation of the liver progenitor cell niche in liver diseases: potential involvement of Wnt and Notch signalling. Gut 59:247-57 (2010). IHC-Fr ; Human . Read more (PubMed: 19880964) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic tooth. Early bell stage.)
Specification Embryonic tooth. Early bell stage.
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 (0.1%)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Gaskon Ibarretxe

Verified customer

提交于 Feb 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"