Anti-LDL Receptor抗体(ab14056)

概述

  • 产品名称Anti-LDL Receptor抗体
    参阅全部 LDL Receptor 一抗
  • 描述
    鸡多克隆抗体to LDL Receptor
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 29-205 of Human LDL Receptor.

  • 阳性对照
    • LDLR transfected 293 cell lysate.

应用

Our Abpromise guarantee covers the use of ab14056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 68, 100 kDa.
This antibody has been tested on recombinant protein only. We have not tested it on endogenous protein.

靶标

  • 功能Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
  • 疾病相关Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
  • 序列相似性Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • 翻译后修饰N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.
  • 细胞定位Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FH antibody
    • FHC antibody
    • LDL R antibody
    • LDL receptor antibody
    • LDLCQ2 antibody
    • Ldlr antibody
    • LDLR_HUMAN antibody
    • Low Density Lipoprotein Receptor antibody
    • Low density lipoprotein receptor class A domain containing protein 3 antibody
    • Low density lipoprotein receptor familial hypercholesterolemia antibody
    • Low-density lipoprotein receptor antibody
    see all

Anti-LDL Receptor antibody 图像

  • Transiently transfected 293 cells probed with ab14056.
  • Anti-LDL Receptor antibody (ab14056) at 1/5000 dilution + CHO-cells transfected with human LDLR at 20 µg

    Secondary
    Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 120,150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-LDL Receptor antibody (ab14056)参考文献

This product has been referenced in:
  • Rojek A  et al. Liver-specific Aquaporin 11 knockout mice show rapid vacuolization of the rough endoplasmic reticulum in periportal hepatocytes after amino acid feeding. Am J Physiol Gastrointest Liver Physiol 304:G501-15 (2013). Mouse . Read more (PubMed: 23275615) »
  • Du F  et al. Novel Domain Interaction Regulates Secretion of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Protein. J Biol Chem 286:43054-61 (2011). Read more (PubMed: 22027821) »

See all 17 Publications for this product

Product Wall

Application Western blot
Sample Human Recombinant protein (CHO-cells transfected with human LDLR)
Loading amount 20 µg
Specification CHO-cells transfected with human LDLR
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Feb 22 2007

Thank you for your enquiry. For ab14056, this was tested against a fusion protein corresponding to amino acids 29 to 205 of the human LDL receptor. Thus, the bands seen will be much smaller than for the full-length protein. Native LDLR will run as...

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Thank you for your email. Neither of these antibodies have been tested yet for application in FACS. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how ...

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Thank you for your enquiry. I have been in touch with the source of this antibody and I have determined that neutralisation is not recommended as an application for this antibody. Although mouse and human LDL-r have good homology in sequence and th...

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Thank you for your enquiry and for taking your time to fill in the Questionnaire. We are very sorry to hear that you are having problem with this antibody. Could you please let us know the batch number you have received? It may well be that the ant...

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I'm sorry to hear ab14056 is giving your customer problems. I have had a good look at the protocol you sent and it looks fine, I would recommend sending him free of charge a new vial to try out. I hope this helps, please let me know if you need furthe...

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Thank you for your email, and I am very pleased to hear that ab14056 is working out for you in both Western blotting and IHC. I suggest that you submit a review (and get a gift certificate to Amazon.com in return!) - just go to the online datasheet for...

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Thank you very much for your patience. I have just received the following information regarding this antibody: ab14056 was raised against a protein domain of 176 amino acids at the N-terminus and is expected to work in IHC. However, this has not yet be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"