The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
LDHD belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. D-lactate and D-glycerate dehydrogenase activities have been found in a similar protein in yeast. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.