概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to LDHA
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human LDHA (NP_005557.1)

  • 阳性对照
    • Human liver tissue lysate, LDHA transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab92903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/500 - 1/1000. Predicted molecular weight: 37 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 通路
      Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • 疾病相关
      Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • 序列相似性
      Belongs to the LDH/MDH superfamily. LDH family.
    • 翻译后修饰
      ISGylated.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase A chain antibody
      • L-lactate dehydrogenase A chain antibody
      • l7R2 antibody
      • Lactate dehydrogenase 1, A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase A4 antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH muscle subunit; M LDH antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • ldha antibody
      • LDHA_HUMAN antibody
      • LDHM antibody
      • OTTMUSP00000017774 antibody
      • PIG19 antibody
      • Proliferation-inducing gene 19 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      see all

    图片

    • Anti-LDHA antibody (ab92903) at 1/500 dilution + Human liver tissue lysate at 50 µg

      Predicted band size : 37 kDa
    • All lanes : Anti-LDHA antibody (ab92903) at 1/500 dilution

      Lane 1 : LDHA transfected 293T cell lysate
      Lane 2 : Untransfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 37 kDa

    实验方案

    文献

    ab92903 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SH-SY5Y cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    SH-SY5Y cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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