LDH-Cytotoxicity Assay试剂盒(Fluorometric) (ab197004)

概述

  • 产品名称
    LDH-Cytotoxicity Assay试剂盒(Fluorometric)
    参阅全部 Lactate dehydrogenase 试剂盒
  • 检测方法
    Fluorescent
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
    Quantitative
  • 灵敏度
    100 cells/well
  • 检测时间
    0h 20m
  • 种属反应性
    与反应: Human
    预测可用于: Mammal
  • 产品概述

    LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004) provides a sensitive, quick, and easy way to detect LDH (lactate dehydrogenase) released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.


    The protocol of this product has been optimized for use on 96-well tissue culture plate, but it can be easily modified to be used with 6-, 12- or 24-well tissue culture plates.


    Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.

  • 说明

    Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydragenase (LDH, EC 1.1.1.27) is a stable enzyme, present in all cell types, which is rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

  • 经测试应用
    适用于: Functional Studiesmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab197004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • Untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

  • Relative fluorescence units of untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

  • Overnight treatment of HeLa cells with 100μM of staurosporine or 3μM of cycloheximide. LDH released into the medium was measured along with blank, untreated cells (low control), LDH Positive Control, and lysed cells (high control)

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab197004.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册