LDH-Cytotoxicity Assay试剂盒(Fluorometric) (ab197004)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 100 cells/well
概述
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产品名称
LDH-Cytotoxicity Assay试剂盒(Fluorometric)
参阅全部 Lactate Dehydrogenase 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Enzyme activity (quantitative) -
灵敏度
100 cells/well -
检测时间
0h 20m -
种属反应性
与反应: Mammals, Other species -
产品概述
LDH Assay Kit (Cytotoxicity, Fluorometric) (ab197004) provides a sensitive, quick, and easy way to detect LDH (lactate dehydrogenase) released from damaged cells.
In the LDH assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm).
The amount of fluorescence is directly proportional to the number of damaged cells.
The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells. 5µl of cell culture media required per assay.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K314 PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit. K314-500 is the same size as the 500 test size of ab197004.
Alternative LDH assays
If you would like to use a fluorometric assay, please refer to LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004).
This kit is more sensitive than the colorimetric LDH Cytoxicity Assay Kit ab65391.
To measure LDH activity in sample types such as serum, plasma, and cell lysates, we recommend LDH assay kit ab102526.
Related products and guides to cytotoxicity / cell viability / proliferation assays
Review our cell health assay guide to learn about our other kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests 500 tests Developer Mix I 1 vial 1 vial LDH Assay Buffer 1 x 50ml 1 x 50ml LDH Positive Control 1 x 20µl 1 x 20µl Lysis Buffer II 1 x 5ml 1 x 5ml PicoProbe III 1 x 2ml 1 x 2ml -
研究领域
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通路
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
疾病相关
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
序列相似性
Belongs to the LDH/MDH superfamily. LDH family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. - Information by UniProt
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别名
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all -
数据库链接
- SwissProt: Q7SI97 Other species
相关产品
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Assay kits
图片
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Untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).
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Relative fluorescence units of untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).
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Overnight treatment of HeLa cells with 100μM of staurosporine or 3μM of cycloheximide. LDH released into the medium was measured along with blank, untreated cells (low control), LDH Positive Control, and lysed cells (high control)
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab197004 被引用在 10 文献中.
- Trinidad-Calderón PA et al. nurP28, a New-to-Nature Zein-Derived Peptide, Enhances the Therapeutic Effect of Docetaxel in Breast Cancer Monolayers and Spheroids. Molecules 27:N/A (2022). PubMed: 35566175
- Xu W et al. Dexmedetomidine pretreatment alleviates ropivacaine-induced neurotoxicity via the miR-10b-5p/BDNF axis. BMC Anesthesiol 22:304 (2022). PubMed: 36163004
- Sun Y et al. TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells. Immun Inflamm Dis 10:e641 (2022). PubMed: 35759233
- Zhang Z et al. Roflumilast, a type of phosphodiesterase 4 inhibitor, can reduce intestinal injury caused by sepsis. Exp Ther Med 22:1398 (2021). PubMed: 34650646
- Du S et al. Does Maternal Stress Affect the Early Embryonic Microenvironment? Impact of Long-Term Cortisol Stimulation on the Oviduct Epithelium. Int J Mol Sci 21:N/A (2020). PubMed: 32284519
- Sharma P et al. Cadmium exposure activates Akt/ERK Signaling and pro-inflammatory COX-2 expression in human gallbladder epithelial cells via a ROS dependent mechanism. Toxicol In Vitro 67:104912 (2020). PubMed: 32512147
- Qi Y et al. Tetrahydroxystilbene glucoside protects against LPS-induced endothelial dysfunction via inhibiting RhoA/ROCK signaling and F-actin remodeling. Gen Physiol Biophys 39:407-417 (2020). PubMed: 33084595
- Ayupova D et al. An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries. Nanomaterials (Basel) 9:N/A (2019). PubMed: 30678192
- Oyama Y et al. Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium. Cell Rep 28:1471-1484.e11 (2019). PubMed: 31390562
- Sillman B et al. Creation of a long-acting nanoformulated dolutegravir. Nat Commun 9:443 (2018). PubMed: 29402886