概述

  • 产品名称
  • 描述
    兔多克隆抗体to LDB1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Fr, WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Pig, Zebrafish
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 50 and 345 of Human LDB1 (NP_003884)

  • 阳性对照
    • WB: HEK293T, A431, HeLa, HepG2 and Raji cell lysates; Mouse brain tissue lysate. IHC-P: U87 xenograft; Mouse muscle and brain tissues. IHC-Fr: Mouse brain tissue. ICC/IF: HeLa cells. IP: HEK293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab96799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
  • 组织特异性
    Expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • 序列相似性
    Belongs to the LDB family.
  • 结构域
    The dimerization domain is located in the N-terminus.
  • 翻译后修饰
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Carboxyl Terminal LIM Domain Binding 2 antibody
    • Carboxyl-terminal LIM domain-binding protein 2 antibody
    • CLIM 2 antibody
    • CLIM-2 antibody
    • hLdb1 antibody
    • LDB-1 antibody
    • ldb1 antibody
    • LDB1_HUMAN antibody
    • LIM Domain Binding 1 antibody
    • LIM domain binding factor CLIM2 antibody
    • LIM domain-binding factor CLIM2 antibody
    • LIM domain-binding protein 1 antibody
    • NLI antibody
    • Nuclear LIM Domain Interactor antibody
    • Nuclear LIM interactor antibody
    • xldb1 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded U87 xenograft using ab96799 at a dilution of 1/100.
  • Anti-LDB1 antibody (ab96799) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 47 kDa

  • Immunofluorescence analysis of paraformaldheyde-fixed HeLa cells using ab96799 at a dilution of 1/200.
  • All lanes : Anti-LDB1 antibody (ab96799) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa

  • Anti-LDB1 antibody (ab96799) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 47 kDa

  • LDB1 was immunoprecipitated from 1000 µg 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab96799. Western blot was performed from the immunoprecipitate using 2.5 μg of ab96799 at 1/1000 dilution.

    Lane A: 20 μg 293T whole cell lysate/extract.
    Lane B: Control with 2.5 μg of preimmune rabbit IgG.
    Lane C: ab96799 IP in 293T (12% SDS-PAGE). 

  • Paraffin-embedded mouse muscle tissue stained for LDB1 using ab96799 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat middle brain tissue stained for LDB1 using ab96799 at a/500 dilution in immunohistochemical analysis.

  • ab96799 staining LDB1 (red) in mouse brain tissue section immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 dilution in PBST) for 12 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/1000 dilution) was used as the secondary antibody.

文献

This product has been referenced in:
  • Stanulovic VS  et al. LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage. Nucleic Acids Res 45:9874-9888 (2017). Read more (PubMed: 28973433) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse Brain)
Specification
Mouse Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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