概述

  • 产品名称
  • 描述
    兔多克隆抗体to LCORL
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: PHSTLEYKVK ERSGTLKTPP KKKLRLPDTG LYMTDSGTGS CRNSSKPV, corresponding to amino acids 470-517 of LCORL

  • 阳性对照
    • Testis lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay was 1:1262500.

靶标

  • 功能
    May act as transcription activator that binds DNA elements with the sequence 5'-CCCTATCGATCGATCTCTACCT-3'. May play a role in spermatogenesis.
  • 序列相似性
    Contains 1 HTH psq-type DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LCoR-like protein antibody
    • LCORL antibody
    • LCORL_HUMAN antibody
    • ligand dependent nuclear receptor corepressor like antibody
    • Ligand-dependent nuclear receptor corepressor-like protein antibody
    • MBLK1 related protein antibody
    • MLR1 antibody
    • Transcription factor MLR1 antibody
    see all

Anti-LCORL antibody 图像

  • Anti-LCORL antibody (ab49420) at 1.25 µg/ml + Testis lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-LCORL antibody (ab49420)参考文献

ab49420 has not yet been referenced specifically in any publications.

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