概述

  • 产品名称
  • 描述
    兔多克隆抗体to LCOR
  • 特异性
    This antibody is specific for Lcor.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide: QQFAAEYTSK TSSTQDPSQP NSTKNQSLPK ASPVTTSPTA ATTQNPVLSK , corresponding to amino acids 6-55 of Mouse LCOR

  • 阳性对照
    • SP2/0 lysate

性能

应用

Our Abpromise guarantee covers the use of ab48339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    May act as transcription activator that binds DNA elements with the sequence 5'-CCCTATCGATCGATCTCTACCT-3' (By similarity). Repressor of ligand-dependent transcription activation by target nuclear receptors. Repressor of ligand-dependent transcription activation by ESR1, ESR2, NR3C1, PGR, RARA, RARB, RARG, RXRA and VDR.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Contains 1 HTH psq-type DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A630025C20RIK antibody
    • C10orf12 antibody
    • FLJ38026 antibody
    • KIAA1795 antibody
    • LCoR antibody
    • LCOR_HUMAN antibody
    • Ligand dependent corepressor antibody
    • Ligand dependent nuclear receptor corepressor antibody
    • Ligand-dependent corepressor antibody
    • Mblk1 related protein 2 antibody
    • Mblk1-related protein 2 antibody
    • MLR2 antibody
    • RP11 175O19.1 antibody
    see all

图片

  • Anti-LCOR antibody (ab48339) at 0.5 µg/ml + SP2/0 lysate with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

实验方案

文献

This product has been referenced in:
  • Viswakarma N  et al. Transcriptional regulation of mixed lineage kinase 3 by estrogen and its implication in ER-positive breast cancer pathogenesis. Oncotarget 8:33172-33184 (2017). ChIP . Read more (PubMed: 28388540) »

See 1 Publication for this product

客户评价及客户问答

Merci de votre intérêt pour les anti-LCOR de chez Abcam.

Après vérification je peux vous confirmer que les deux anticorps ab48339 et ab105825 sont exactement les mêmes. La seconde référence a &eac...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
50 µg
Specification
HCT116
Gel Running Conditions
Non-reduced Denaturing (10% Acrylamide)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Loading amount
50 µg
Specification
HEK293T
Gel Running Conditions
Non-reduced Denaturing (10% Acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Sep 30 2011

Application
Western blot
Sample
Monkey Cell lysate - whole cell (COS7)
Loading amount
50 µg
Specification
COS7
Gel Running Conditions
Non-reduced Denaturing (10% Acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: TA°C
Username

Abcam user community

Verified customer

提交于 Sep 30 2011

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK293T)
Total protein in input
50 µg
Specification
HEK293T
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

提交于 Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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