概述

  • 产品名称Anti-Lck抗体[Y123]
    参阅全部 Lck 一抗
  • 描述
    兔单克隆抗体[Y123] to Lck
  • 特异性This antibody is specific for human Lck, it does not cross react with any other SRC family members. WB results were negative for the following Mouse and Rat tissues/cell lines: Ms brain, heart, kidney, spleen, C6, Raw264.7, PC12, NIH3T3, Ms thymus and serum.
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues before the SH3 domain of Human Lck.

  • 表位ab32149 reacts with an epitope located in the region before SH3 domain of Lck.
  • 阳性对照
    • Jurkat cell lysate. human lymphoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 58 kDa.
IHC-P Use at an assay dependent concentration.
ICC 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/200.

靶标

  • 功能Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
  • 组织特异性Expressed specifically in lymphoid cells.
  • 疾病相关Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 结构域The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
  • 翻译后修饰Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
  • 细胞定位Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
  • Information by UniProt
  • 数据库链接
  • 形式This protein is known to be similar in amino acid sequence to HCK (P08631), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 别名
    • IMD22 antibody
    • LCK antibody
    • Lck p56 antibody
    • LCK proto-oncogene, Src family tyrosine kinase antibody
    • LCK_HUMAN antibody
    • Leukocyte C-terminal Src kinase antibody
    • LSK antibody
    • Lymphocyte cell specific protein tyrosine kinase antibody
    • Lymphocyte cell-specific protein-tyrosine kinase antibody
    • Lymphocyte specific protein tyrosine kinase antibody
    • Membrane associated protein tyrosine kinase antibody
    • Oncogene lck antibody
    • P56 LCK antibody
    • p56(LSTRA) protein tyrosine kinase antibody
    • p56-LCK antibody
    • p56lck antibody
    • pp58 lck antibody
    • pp58lck antibody
    • Protein YT16 antibody
    • Proto oncogene tyrosine protein kinase LCK antibody
    • Proto-oncogene Lck antibody
    • Protooncogene tyrosine protein kinase LCK antibody
    • T cell specific protein tyrosine kinase antibody
    • T cell-specific protein-tyrosine kinase antibody
    • T lymphocyte specific protein tyrosine kinase p56lck antibody
    • Tyrosine-protein kinase Lck antibody
    • YT 16 antibody
    • YT16 antibody
    see all

Anti-Lck antibody [Y123] 图像

  • Anti-Lck antibody [Y123] (ab32149) at 1/1000 dilution + Jurkat cell lysate.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab32149 at a 1/250 dilution staining Lck in human lymphoma using Immunohistochemistry, Paraffin Embedded Tissue.
  • Overlay histogram showing Jurkat cells stained with ab32149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32149, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Lck antibody [Y123] (ab32149)参考文献

This product has been referenced in:
  • Zhu L  et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »
  • Obr A  et al. Real-time analysis of imatinib- and dasatinib-induced effects on chronic myelogenous leukemia cell interaction with fibronectin. PLoS One 9:e107367 (2014). WB ; Human . Read more (PubMed: 25198091) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MKN45 cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification MKN45 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Verified customer

提交于 Oct 21 2016

Application Western blot
Sample Mouse Tissue lysate - whole (hippocampus)
Loading amount 25 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"