Anti-LCAT抗体[EPR1383(2)] (ab109417)


  • 产品名称Anti-LCAT抗体[EPR1383(2)]
    参阅全部 LCAT 一抗
  • 描述
    兔单克隆抗体[EPR1383(2)] to LCAT
  • 经测试应用适用于: ICC, WB, IP, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human LCAT.

  • 阳性对照
    • Human serum and Human plasma lysates; Human liver tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
    • 组织特异性Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. In liver, expressed in HEPG2 hepatocytes.
    • 疾病相关Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]; also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure.
      Defects in LCAT are a cause of fish-eye disease (FED) [MIM:136120]; also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye').
    • 序列相似性Belongs to the AB hydrolase superfamily. Lipase family.
    • 翻译后修饰O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures.
    • 细胞定位Secreted. Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LCAT antibody
      • LCAT_HUMAN antibody
      • Lecithin cholesterol acyltransferase antibody
      • Lecithin-cholesterol acyltransferase antibody
      • Phosphatidylcholine sterol acyltransferase antibody
      • Phosphatidylcholine-sterol acyltransferase antibody
      • Phospholipid cholesterol acyltransferase antibody
      • Phospholipid-cholesterol acyltransferase antibody
      see all

    Anti-LCAT antibody [EPR1383(2)] 图像

    • All lanes : Anti-LCAT antibody [EPR1383(2)] (ab109417) at 1/1000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 50 kDa
    • ab109417, at 1/250 dilution, staining LCAT in paraffin-embedded Human liver tissue by Immunohistochemistry.

    Anti-LCAT antibody [EPR1383(2)] (ab109417)参考文献

    ab109417 has not yet been referenced specifically in any publications.

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