兔多克隆抗体to LC3C - N-terminal
Synthetic peptide within Human LC3C (N terminal). The exact sequence is proprietary. Carrier-protein conjugated
Database link: Q9BXW4
U-87MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.025% Proclin Constituents: PBS, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Most abundant in placenta, lung and ovary.
Belongs to the MAP1 LC3 family.
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
Information by UniProt
Autophagy-related protein LC3 C antibody
Autophagy-related ubiquitin-like modifier LC3 C antibody
Western blot - Anti-LC3C antibody - N-terminal (ab229667)
All lanes :
Anti-LC3C antibody - N-terminal (ab229667) at 1/1000 dilution
Lane 1 :
U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 :
SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 :
IMR32 (Human brain neuroblast cell line) whole cell extract
Lane 4 :
SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size :
15% SDS-PAGE gel.
has not yet been referenced specifically in any publications.
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