概述

  • 产品名称
  • 描述
    兔多克隆抗体to LC3C
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 1-30 (MPPPQKIPSVRPFKQRKSLAIRQEEVAGIR) of Human LC3C (UniProt ID: Q9BXW4).

  • 阳性对照
    • WB: 293, Ramos, HeLa, HepG2, T47D, and Jurkat cell lysates.
      IHC: Human cancer tissue

性能

应用

Our Abpromise guarantee covers the use of ab168813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • 组织特异性
    Most abundant in placenta, lung and ovary.
  • 序列相似性
    Belongs to the MAP1 LC3 family.
  • 翻译后修饰
    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • 细胞定位
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATG8J antibody
    • Autophagy-related protein LC3 C antibody
    • Autophagy-related ubiquitin-like modifier LC3 C antibody
    • LC3 like protein 2 antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3 like protein 3 antibody
    • MAP1 light chain 3-like protein 3 antibody
    • MAP1A/MAP1B LC3 C antibody
    • MAP1A/MAP1B light chain 3 C antibody
    • MAP1LC3C antibody
    • Microtubule-associated protein 1 light chain 3 gamma antibody
    • Microtubule-associated proteins 1A/1B light chain 3C antibody
    • MLP3C_HUMAN antibody
    see all

图片

  • All lanes : Anti-LC3C antibody (ab168813) at 1/100 dilution

    Lane 1 : 293 lysate
    Lane 2 : Ramos lysate
    Lane 3 : HeLa lysate
    Lane 4 : HepG2 lysate
    Lane 5 : T47D lysate
    Lane 6 : Jurkat tissue lysate

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 17 kDa
  • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded Human breast carcinoma tissue labeling LC3C with ab168813 at 1/50.

文献

ab168813 has not yet been referenced specifically in any publications.

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