概述

  • 产品名称Anti-LC3B抗体
    参阅全部 LC3B 一抗
  • 描述
    兔多克隆抗体to LC3B
  • 特异性ab51520 is specific for the B isoform of LC3.
  • 经测试应用适用于: IHC - Wholemount, Flow Cyt, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to the N terminal region of human LC3, isoform B protein.
    Database link: Q9GZQ8

  • 阳性对照
    • WB: Treated U87-MG lysates. ICC/IF: Treated U87-MG cells. human malignant brain tumors, breast cancer, leukemia samples and murine cells
  • 常规说明

    ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.

性能

应用

Our Abpromise guarantee covers the use of ab51520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount Use at an assay dependent concentration.
Flow Cyt 1/200.
IHC-Fr 1/2000.
WB 1/3000. Predicted molecular weight: 15 kDa. Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I).
ICC/IF 1/2000.

靶标

  • 功能Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • 组织特异性Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • 序列相似性Belongs to the MAP1 LC3 family.
  • 翻译后修饰The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • 细胞定位Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATG8F antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3B antibody
    • LC3II antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1LC3B a antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Anti-LC3B antibody 图像

  • All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution

    Lane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 0 µM
    Lane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 2 µM
    Lane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 4 µM
    Lane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 8 µM


    Predicted band size : 15 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

  • Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
    1) Control
    2) Temozolomide
  • Flow cytometry analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.

  • Ab51520 at 1/2000 dilution staining treated U373-MG cells;
    1) Control
    2) C2-ceramide.
    The nuclei were stained with DAPI.
  • Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
    1) Control
    2) Temozolomide
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

  • Ab51520 at 1/2000 dilution staining gliobastoma multiform tissue
    1) normal brain
    2) gliobastoma multiform tissue
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).

Anti-LC3B antibody (ab51520)参考文献

This product has been referenced in:
  • Ruiz A  et al. Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis. Cell Death Dis 7:e2059 (2016). IHC-P ; Mouse . Read more (PubMed: 26775710) »
  • Xu TX  et al. Hypoxia responsive miR-210 promotes cell survival and autophagy of endometriotic cells in hypoxia. Eur Rev Med Pharmacol Sci 20:399-406 (2016). WB ; Human . Read more (PubMed: 26914112) »

See all 20 Publications for this product

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Application Immunocytochemistry
Sample Mouse Cultured Cells (Cardiomyocytes)
Permeabilization Yes - Triton x-100, 0.01%
Specification Cardiomyocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Paraformaldehyde
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提交于 Dec 03 2015

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Heart)
Permeabilization Yes - Triton x-100, 0.01%
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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提交于 Nov 06 2015

Application Flow Cytometry
Sample Mouse Cell (Cardiomyocytes)
Permeabilization Yes - Triton X-100, 0.01%
Gating Strategy LC3B-AlexaFluor488 positive
Specification Cardiomyocytes
Preparation Cell harvesting/tissue preparation method: Trypsinization
Sample buffer: 0.25% for 30 sec
Fixation Paraformaldehyde
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提交于 Sep 30 2015

Application Western blot
Sample Human Cell lysate - whole cell (Heart)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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提交于 Sep 30 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Cardiomyocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 28 2015

Application Immunocytochemistry
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Sample Human Cultured Cells (HEK293)
Specification HEK293
Permeabilization Yes - Triton x-100, 0.01%
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Jan 16 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Mouse Cell (RAW cell)
Specification RAW cell
Permeabilization Yes - 3% BSA in PBS with 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

提交于 Nov 13 2013

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

提交于 Nov 13 2013

The antibody's immunogen is over 95% identical to the homologous sequence in zebrafish. Since this is a polyclonal antibody, and is assumed to recognize multiple epitopes, it is likely that it recognizes epitopes that are 100% identical in the immunoge...

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