概述

  • 产品名称
  • 描述
    兔多克隆抗体to LC3B
  • 特异性
    ab51520 is specific for the B isoform of LC3.
  • 经测试应用
    适用于: IHC - Wholemount, Flow Cyt, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to the N terminal region of human LC3, isoform B protein.
    Database link: Q9GZQ8

  • 阳性对照
    • WB: Treated U87-MG lysates. ICC/IF: Treated U87-MG cells. human malignant brain tumors, breast cancer, leukemia samples and murine cells
  • 常规说明

    ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.

    The product formulation changed to include 1% BSA on 17th May 2017. The following lot does not contain BSA and is still in stock GR320876. All future lots will contain 1% BSA.

性能

应用

Our Abpromise guarantee covers the use of ab51520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount Use at an assay dependent concentration.
Flow Cyt 1/200.
IHC-Fr 1/2000.
WB 1/3000. Predicted molecular weight: 15 kDa. Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I).
ICC/IF 1/2000.

靶标

  • 功能
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • 组织特异性
    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • 序列相似性
    Belongs to the MAP1 LC3 family.
  • 翻译后修饰
    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • 细胞定位
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATG8F antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3B antibody
    • LC3II antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1LC3B a antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Anti-LC3B antibody 图像

  • All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution

    Lane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 0 µM
    Lane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 2 µM
    Lane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 4 µM
    Lane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 8 µM


    Predicted band size : 15 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

  • Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
    1) Control
    2) Temozolomide
  • Flow cytometry analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.

  • Ab51520 at 1/2000 dilution staining treated U373-MG cells;
    1) Control
    2) C2-ceramide.
    The nuclei were stained with DAPI.
  • Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
    1) Control
    2) Temozolomide
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

  • Ab51520 at 1/2000 dilution staining gliobastoma multiform tissue
    1) normal brain
    2) gliobastoma multiform tissue
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).

Anti-LC3B antibody (ab51520)参考文献

This product has been referenced in:
  • Wang X  et al. Role of hypoxia-inducible factor-1a in autophagic cell death in microglial cells induced by hypoxia. Mol Med Rep 15:2097-2105 (2017). Read more (PubMed: 28259912) »
  • Cho JH  et al. Downregulation of SIRT1 signaling underlies hepatic autophagy impairment in glycogen storage disease type Ia. PLoS Genet 13:e1006819 (2017). WB . Read more (PubMed: 28558013) »

See all 27 Publications for this product

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Regrettably, this antibody is not in stock at the moment. Another batch is in production and is expected to be available by the...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cardic stem celll)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardic stem celll
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 16 2016

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 03 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Heart)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 06 2015

Application
Flow Cytometry
Sample
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - Triton X-100, 0.01%
Gating Strategy
LC3B-AlexaFluor488 positive
Specification
Cardiomyocytes
Preparation
Cell harvesting/tissue preparation method: Trypsinization
Sample buffer: 0.25% for 30 sec
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 30 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Sep 30 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Sample
Human Cultured Cells (HEK293)
Specification
HEK293
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (RAW cell)
Specification
RAW cell
Permeabilization
Yes - 3% BSA in PBS with 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

提交于 Nov 13 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Tissue sections (Uterus)
Specification
Uterus
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

提交于 Nov 13 2013

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