概述

  • 产品名称Anti-LC3A/B抗体
    参阅全部 LC3A/B 一抗
  • 描述
    兔多克隆抗体to LC3A/B
  • 特异性Recognizes human and rat LC3A/B-I and LC3A/B-II by immunoblotting and immunoprecipitation (~18 kDa and ~16 kDa, respectively). Recognizes mouse LC3A/B-I (~18 kDa). The detection of mouse LC3A/B-II may require specific cell treatment and/or long exposure.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human LC3A/B aa 36-49 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Also SwissProt ID: Q9GZQ8
    Sequence:

    ERYKGEKQLPVLDK


    Database link: Q9H492

  • 阳性对照
    • Whole brain. U87 cells.

应用

Our Abpromise guarantee covers the use of ab62721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).
IP Use at an assay dependent dilution.

靶标

Anti-LC3A/B antibody 图像

  • Lane 1 : Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml
    Lanes 2 - 3 : Anti-LC3A/B antibody (ab62721) at 1 µg/ml

    Lane 1 : Rat whole brain extract
    Lane 2 : Rat whole brain extract
    Lane 3 : Rat whole brain extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate
    Developed using the ECL technique

    Predicted band size : 16 - 18 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

Anti-LC3A/B antibody (ab62721)参考文献

This product has been referenced in:
  • Gao H  et al. Rho-Kinase inhibitor fasudil suppresses high glucose-induced H9c2 cell apoptosis through activation of autophagy. Cardiovasc Ther 34:352-9 (2016). Read more (PubMed: 27333569) »
  • Wang J  et al. Cerebral ischemic post-conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation cellular model. Mol Med Rep 14:4162-4172 (2016). WB ; Rat . Read more (PubMed: 27666823) »

See all 9 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (lung)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 35 µg
Specification lung
Blocking step BSA as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 May 19 2017

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (COV434 cell line)
Specification COV434 cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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提交于 Feb 13 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Mouse Tissue lysate - whole (Muscle)
Specification Muscle
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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提交于 Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"