Anti-Lbx2抗体(ab76728)

概述

性能

应用

Our Abpromise guarantee covers the use of ab76728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 21 kDa.

靶标

  • 功能Putative transcription factor.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ladybird homeobox 2 antibody
    • Ladybird homeobox homolog 2 (Drosophila) antibody
    • Ladybird homeobox homolog 2 antibody
    • Ladybird homeobox protein homolog 2 antibody
    • Ladybird like homeobox 2 antibody
    • Ladybird like homeodomain protein 2 antibody
    • Lbx 2 antibody
    • Lbx2 antibody
    • LBX2_HUMAN antibody
    • LP3727 antibody
    • RGD1561172 antibody
    • Transcription factor LBX2 antibody
    see all

Anti-Lbx2 antibody 图像

  • Anti-Lbx2 antibody (ab76728) at 1 µg/ml + Recombinant tagged human Lbx2 fragment at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 21 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
  • ab76728, at 10µg/ml, staining Lbx2 in Hela cells by immunofluorescence.
  • Immunofluorescence of sand lizards muscles progenitor cells in limb bud labeling Lbx2 with ab76728. Fixed with paraformaldehyde, permeabilised with Tween 20 abd blocked with 50µg/ml of BSA for 1 hour and room tempreture. A Cy5® donkey anti-mouse IgG polyclonal antibody was used as a secondary (1/500).

    See Abreview

Anti-Lbx2 antibody (ab76728)参考文献

ab76728 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Sand lizard Tissue sections (muscles progenitor cells in limb bud)
Permeabilization Yes - Tween 20
Specification muscles progenitor cells in limb bud
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50µg/mL · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 07 2017

Thank you for your reply.

Yes, you are right, you need to purchase the antibody first.
Abcam does not ship directly to Argentina.
To place an order, please contact our distributor, detailsare viathis link: http://www.abcam.com/index.ht...

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Thank you very much for your interest in ab76728.

To our knowledge,ab76728 has not been tested in Mouse (79% homologie between the immunogen and the Mouse Lbx2, see attachment). Therefore, I can offer a discount off a future purchase if you bu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"