概述

  • 产品名称Anti-LATS2抗体
    参阅全部 LATS2 一抗
  • 描述
    兔多克隆抗体to LATS2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human LATS2.

  • 阳性对照
    • This antibody gave a positive signal in Human Ovary and Human Testis tissue lysates as well as the following whole cell lysates: HeLa; HepG2; MCF7. It also gave a positive signal in FFPE human heart tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab110780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
  • 组织特异性Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 翻译后修饰Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ13161 antibody
    • Kinase phosphorylated during mitosis protein antibody
    • KPM antibody
    • Large tumor suppressor homolog 2 antibody
    • Large tumor suppressor homolog 2 Drosophila antibody
    • Large tumor suppressor kinase 2 antibody
    • LATS large tumor suppressor Drosophila homolog 2 antibody
    • LATS large tumor suppressor homolog 2 antibody
    • Lats2 antibody
    • LATS2_HUMAN antibody
    • Serine/threonine kinase kpm antibody
    • Serine/threonine protein kinase kpm antibody
    • Serine/threonine-protein kinase kpm antibody
    • Serine/threonine-protein kinase LATS2 antibody
    • Warts like kinase antibody
    • Warts-like kinase antibody
    see all

Anti-LATS2 antibody 图像

  • All lanes : Anti-LATS2 antibody (ab110780) at 1 µg/ml

    Lane 1 : Human ovary tissue lysate - total protein (ab30222)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 120 kDa
    Additional bands at : 65 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of ab110780 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110780, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-LATS2 antibody (ab110780)参考文献

ab110780 has not yet been referenced specifically in any publications.

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